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January 13, 2015

Thailand

LAMP Assay Superior to PCR in Detecting AHPND (EMS)

 

From Abstract: In this study, two sets of primers (LAMP-A2 and LAMP-A3) were developed and validated for use in a LAMP assay to specifically identify the Vibrio parahaemolyticus strain that causes AHPND.   LAMP-A2 and LAMP-A3 detected all 33 V. parahaemolyticus AHPND isolates except the non-V. parahaemolyticus AHPND isolates and 19 other closely related bacterial species.  In pure culture and in spiked shrimp experiments, the LAMP assay was superior to PCR for the detection of V. parahaemolyticus AHPND.  In pure cultures, the detection limit of LAMP-A3 was ten times lower that that of LAMP-A2.  Water samples collected from a shrimp farm revealed that V. parahaemolyticus AHPND was detected mostly in sediment samples.  Taken together, the results suggested that the LAMP-A3-based LAMP assay, but not LAMP-A2 or PCR, was suitable for the identification of V. parahaemolyticus AHPND in shrimp aquaculture.

 

Source: Aquaculture International.  LAMP Assay to Detect Vibrio Parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease in Shrimp.  Jetnaphang Kongrueng, Natta Tansila, Pimonsri Mitraparp-arthorn, Mitsuaki Nishibuchi, Gary J. Vora and Varaporn Vuddhakul (email varaporn.v@psu.ac.th, Food Safety and Health Research Unit, Department of Microbiology, Faculty of Science, Prince of Songkhla University, Hat Yai, Songkhla, Thailand).  January 7, 2015.

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