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September 16, 2013


Reproductive Performance of Triploid and Diploid Tiger Shrimp


From Abstract: In this study, triploid and diploid giant tiger shrimp (Penaeus monodon) siblings of both sexes were reared to reproductive maturity, crossed with wild-caught females and males and conditioned for spawning.  Then, a comprehensive reproductive performance trial was undertaken. Ovarian development, spawning frequency, fecundity, hatch rate, gonad morphology and male reproductive tracts were assessed.  After ablation, ovarian development and cycling between wild generation zero (G-0) diploids and G-1 diploids was not significantly different, whereas G-1 triploids failed to show any signs of ovarian development and cycling, thus resulting in no G-1 triploid female spawnings.  There were ten G-0 diploid female crosses with G-0 diploid male first-spawnings and nine G-0 diploid female crosses with G-1 diploid male first-spawnings, all of which produced viable nauplii.  In comparison, there were seven G-0 diploid female crosses with G-1 triploid male first-spawnings, none of which produced viable nauplii.  The 26 wild G-0 diploid female spawnings had more eggs than the one G-1 diploid female spawning.  Gonad morphology and male reproductive tract assessments showed impaired reproductive development in triploid gonadal tissues of both sexes (compared with sibling diploids and wild shrimp) to a point where complete maturation had not occurred.  The thelycum of sixteen wild G-0 diploid females crossed with G-1 triploid males had no visible spermatophore present, suggesting that G-1 triploid males are incapable of developing viable spermatophores and mating with females.


This study demonstrates that the triploid females and males are incapable of producing viable gametes and are thus reproductively sterile [Emphasis by Shrimp News].


Shrimp News emailed Dr. Melony Sellars, the lead author of the above study, to check on some of the terminology in her abstract.  In her response, Sellars wrote: “Proof-of-concept that triploid induction can confer reproductive sterility and prevent the production of progeny has now been achieved.  The next stage of this research is up-scaling of methodologies for triploid shrimp production to enable commercial adoption.”


Sources: 1. Aquaculture Research.  Reproductive Performance and Mature Gonad Morphology of Triploid and Diploid Black Tiger Shrimp (Penaeus monodon) Siblings.  Melony Sellars (email, CSIRO Food Futures National Research Flagship, CSIRO Marine and Atmospheric Research, Dutton Park, Queensland 4102, Australia), Andrew Wood, Brian Murphy, Greg Coman, Stuart Arnold, Russell McCulloch and Nigel Preston.  Volume 44, Issue 10, Page 1493,  September 2013.  2. Email to Shrimp News International from Melony Sellars.  Subject: RE-Checking on a Couple of Things.  September 15, 2013.


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